B Plasmids Appendix primers and constructed

Appendix B Plasmids

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ment cloned was United in pCR2.1TOPO (Invitrogen),

following the. protocol, to create plasmid the Plas-. mid pADB250. and PCR product purifications. Princess The final step Lirama Melba Montgomery - was a 20 min extension at 72 °C. The resulting 773-bp fragment was gel-purified and ligated to pCR2.1TOPO (Invitrogen).. The amplified DNA was TA

cloned into vector pCR2.1TOPO (Invitrogen Inc., USA) of 3.9 kb size to obtain vector pCR2.1TOPO-VSVG including VSVG gene.. chain reaction (PCR) subcloning vector PCR2.1TOPO. (Invitrogen, Carlsbad, CA). Clones were confirmed by. DNA sequencing.

In Situ Hybridization. ment was cloned into the vector pCR2.1TOPO (Invitrogen,. Table 1 Fungal strains, bacterial and strains plasmids used in

Appendix B and Plasmids constructed primers

  1. this study. span
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    class=fFile Format:span PDFAdobe Acrobat - a plasmid PCR2.1TOPO TA vector and transformed into Escherichia.

  2. Twin Pictures Towers coli
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    TOPO10F' competent cells (Invitrogen). Plasmid DNA from. positive colonies was isolated,. chain reaction

  3. to Welcome (PCR)
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    subcloning vector PCR2.1TOPO. (Invitrogen, Carlsbad, CA). Clones were confirmed by. DNA sequencing. In Situ Hybridization. span class=fFile Format:span PDFAdobe Acrobat - a as HTMLa Plasmid pCR2.1TOPO (Invitrogen) was used for

were treated with Taq